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KMID : 0644120090220010001
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Journal of Oriental Gynecology
2009 Volume.22 No. 1 p.1 ~ p.14
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Neuroprotective Effect of Insamyangyung-tang
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Kim Seung-Hyun
Lee Chang-Hoon
Lee Jin-Moo
Cho Jung-Hoon
Jang Jun-Bock
Lee Kyung-Sub
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Abstract
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Purpose: Oxidative stress was thought to play a critical role in neurodegenerative disease. Many in vivo and in vitro reports explained the possible pathway of human aging. But in therapeutic aspects, there was no clear answers to prevent aging associated with neural diseases. In this study, we investigated the antioxidant and neuroprotective effects of the Insamyangyung-tang (IYT).
Methods: To estimate the antioxidant effects, we carried out 1.1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay, 2,2’-azinobis-(3- ethylbenzothiazoline-6- sulfonic acid (ABTS) radical cation decolorization assay, and measurement of total polyphenolic content. To evaluate neuroprotective effect of IYT in vitro. We performed thiazolyl blue tetrazolium bromide (MTT) assay, reactive oxygen species (ROS) creation in SH-SY5Y. Tyrosine hydroxylase (TH) immunocytochemistry, nitric oxide (NO) assay, and TNF- assay in primary rat mesencephalic dopaminergic neurons.
Results: The values were and in DPPH and ABTS assay respectively. Total polyphenolic content was 1.05%. In SH-SY5Y culture, IYT significantly increased the decreased cell viability by 6-OHDA at the concentrations of in pre-treatment group, in post-treatment group, and in co-treatment group. The production of ROS induced by 6-OHDA was significantly inhibited in IYT treated group. In mesencephalic dopaminergic cell culture, the IYT group reduced the dopaminergic cell loss against 6-OHDA toxicity and the production of No and TNF- at the concentration of .
Conclusion: These results showed that IYT has antioxidant and neuroprotectctive effects in the dopaminergic cells through decreasing the production of ROS, NO and TNF- which can cause many neurodegenerative changes in brain cell.
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KEYWORD
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Insamyangyung-tang, Antioxidative, Neuroprotectctive effect, Herbs, ROS
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